KINETIC STUDIES OF ERYTHROCYTES ACETYLCHOLINESTERASE WITH OTHER DIAGNOSTIC PARAMETERS IN CHRONIC RENAL FAILURE
Keywords:
Enzyme, Acetylcholinesterase, Urea, Creatinine, Chronic, Renal failureAbstract
Objective: To describe the relationship between an Enzyme acetyl cholinesterase (Ach.E, EC. 3.1.1.7) activity and
other diagnostic parameters (urea, creatinine ) used for diagnosing renal failure.
Material and Methods: The activity of the enzyme Ach.E of different ages (both males & females) was estimated in
about 200 admitted renal failure patients along with other diagnostic parameters (urea and creatinine) in the
nephrology unit of P.G.M.I. Lady Reading Hospital, Peshawar, by using Michaelis Menten kinetic parameters aKm
(which shows the affinity of the enzyme towards the substrate) and aVm (which shows the rate of reaction ) in pre
dialyzed blood of patients suffering from chronic renal failure by the method of Ellman Gl. at 30C0 and pH 7.4, using
acetylthiocholine iodide as a substrate. The activity of the enzyme was consolidated by a computerized program.
Results: The parameters (aKm & aVm) were found to be different from the normal in pre dialysed blood and activity
of the enzyme was also low in all cases. A comparative set up was established among urea, creatinine, Hb% and
activity of the enzyme Ach.E. The values of aKm, aVm, urea, creatinine, Hb and activity of the enzyme were 22,77,45,1.5
and 100% respectively in normal individuals, while these values were changed according to the severity of the
diseases, resulting low activity of the enzyme.
Conclusion: This enzymatic technique has an advantage to show us, the activity of Acetylcholinesterase active site
is externally oriented in the membrane of red blood cells, therefore any change occurring in the vicinity of the
membrane will affect the activity of enzyme. This change can be used as a marker for the diagnosis and prognosis of
disease.
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