DEPLETION OF FACTOR EIGHT (VIII) LEVEL IN FRESH FROZEN PLASMA AFTER ONE MONTH STORAGE
Keywords:
Volunteer, Blood Donors, Fresh Frozen, Plasma, Factor VIIIAbstract
Objective: To evaluate the efficacy and quality of fresh frozen plasma after one month storage.
Material and Method: It was a descriptive cross sectional study conducted in blood bank of Hayatabad Medical Complex
Hospital Peshawar,Pakistan. In this study a total number of one hundred and thirty nine (Male n= 121, Female
n= 18) healthy volunteer blood donors were enrolled. Whole blood (450 ml ± 10%) was collected from all donors
into triple packs blood bag (JMS, Germany). The blood components were prepared within 8 hours in a 2-step spin
protocol. Whole blood was centrifuged at 4 0C with low spin (2500 rpm for 10 minutes) and platelets rich plasma was
transferred into 2nd pack of triple bag followed by a high spin for 10 minutes at 3200 rpm. Platelets poor plasma (PPP)
of 2nd pack was transferred into 3rd pack and fresh plasma was stored at -70 0C. Factor VIII assay was performed on
freshly prepared FFPs and repeated after 30 days of storage on Stago STA compact Hemostasis analyzer according
to manufacturer instructions. Data was analyzed by SPSS-20 and presented as mean ± standard deviation.
Results: Total number of 139 healthy blood donors was enrolled in the present study. Majority of blood donors age
was <20 years while only 1.4% blood donors were in the age of 60-70 years, with mean age of 31 years. Out of total
donors 121 (87.1%) were male while 18 (12.9%) were female. In more than 95 % of day 1st FFP unites, level of factor
VIII greater was 50%. On day 30th factor VIII level was decreased by 23% on average in 95 present of unites.
Conclusion: There is sufficient levels of relevant coagulation factor activity present in the plasma obtained from whole
blood with in 8 hours of collection and stored at -18°C and would be an acceptable product for patients requiring FFP
for bleeding disorders for one mont.
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