THE IMPORTANCE OF AUTOMATED HEMATOLOGY ANALYZER WITH MULTI ANGLED POLARIZED SCATTER SEPARATION (MAPSS) BASED FLOW CYTOMETRY
Keywords:
Multi Angled Polarized Scatter Separation, Malaria,, Plasmodium, Vivax, FalciparumAbstract
Objective: To evaluate the efficacy of Multi Angled Polarized Scatter Separation (MAPSS) based flow cytometric
principles for detection of malaria by detecting malarial pigment hemozoin.
Materials and Methods: This study was carried out North-West General Hospital and Research Centre Peshawar
from July 2011 to Sepstember 2011. A toal of 10032 patients were included in the study. Complete blood counts
were done on automated hematology analyzer, Abbott CELL-DYN RUBY. Comparison Multi Angled Polarized Scatter
Separation (MAPSS) based flow cytometry detection of hemozoin containing leukocytes was done with thin and thick
film microscopy of giemsa stained smear.
Results: Total of 144 (1.43 %) cases of malaria were diagnosed on microscopy. 132 cases were vivax species (91.66%)
and remaining 12 cases (8.3 %) were falciparum species. The Multi Angled Polarized Scatter Separation (MAPSS)
flagged 104 cases. (72.2 %) , of which 40 cases could be typed as 38 (95 %) of plasmodium vivax and 2cases (5%)
plasmodium falciparum.
Conclusion: The introduction of automated hematology analyzer having Multi Angled Polarized Scatter Separation
(MAPSS) based flow cytometric principle for detection of malarial pigment hemozoin is of great importance in malaria
endemic countries. This reduces the chances of missing diagnoses and in clinically unsuspected cases where
microscopy for malaria parasite has not been advised.. It also reduces time of cumbersome microscopy when
malaria is not flagged.
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