ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF PSEUDOMONAS AERUGINOSA IN PATIENTS WITH SEPSIS: A CROSS-SECTIONAL STUDY CONDUCTED AT A TERTIARY CARE HOSPITAL IN PAKISTAN
DOI:
https://doi.org/10.52764/jms.26.34.3.10Abstract
Objective: To determine the frequency of Pseudomonas aeruginosa among wound isolates from patients with sepsis and to assess its antimicrobial susceptibility pattern at a tertiary care hospital in Peshawar.
Methods: We conducted a cross-sectional study in the Pathology Department of Lady Reading Hospital, Peshawar, from March to June 2026. We aseptically collected 43 wound samples under sterile conditions from patients with sepsis. We characterized clinical isolates using standard microbiological techniques, including Gram staining, biochemical tests (catalase, coagulase, oxidase, indole, sugar fermentation, and TSI), and colony morphology. We performed antimicrobial susceptibility testing using the Kirby-Bauer disc diffusion method in accordance with Clinical and Laboratory Standards Institute guidelines. Data were analyzed using descriptive statistics.
Results: Of 43 specimens, 4 cases (9.30%) were identified as having Pseudomonas aeruginosa infections. The distribution of P. aeruginosa infection was equal between males and females, with the highest rate observed in the 61 to 80 years age group. Among all tested antibiotics, Amikacin showed the highest sensitivity against P. aeruginosa (100%), whereas ceftriaxone, amoxicillin-clavulanic acid, and tigecycline were completely resistant (100%). In general, the predominant bacteria were Gram-negative, with 31 isolates (72.1%), including Proteus spp. (32.5%), ESBL-producing E. coli (16.2%), and Pseudomonas aeruginosa (9.3%). Gram-positive organisms included 12 isolates (27.9%), with Staphylococcus aureus as the most prevalent type (20.9%).
Conclusion: Pseudomonas aeruginosa was present in 9.3% of wound samples from patients with clinically diagnosed sepsis, with amikacin showing the highest susceptibility.
Keywords: Pseudomonas aeruginosa, Sepsis, Antimicrobial resistance, susceptibility testing
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Copyright (c) 2026 Sadaf Naz, Muhammad Nabi, Fida Hassan, Muhammad Saleh Faisal, Muhammad Mudassir, Maab Ahmad
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