5. ERYTHROCYTE REOLOGICAL PROPERTIES IN CHIDREN WITH PERTHES` DISEASE AND TRANSIENT SYNOVITIS OF THE HIP JOINT
Tauseef Raza1, Sergei Borisivich Nazarov2, Olga Alexandrovna Pahrova3, Aleksei Valerivich Philosophov4
1Department of Orthopaedics, KMU Institute of Medical Sciences, Kohat - Pakistan
2Department of Physiology, Ivanovo State Medical Academy, Russia
3Department of Rheology Laboratory, Scientific Research Center, Ivanovo State Medical Academy, Russia
4Department of Orthopaedic, Children Clinical Hospital No 1, Ivanovo, Russia
Objective: To study parameters of aggregation and cytoarchitecture of erythrocytes in children with Perthes disease
(PD) and Transient synovitis of the hip joint(TS).
Material and Methods: This study was conducted at Children Clinical Hospital No 1, Ivanovo, Russia from 01.11.2001
to 10.02.2006. A total of one hundred and thirty six children (sixteen children from control group), and one hundred and
twenty one children diagnosed as PD and TS were examined. Determination of aggregation parameters of erythrocytes
was carried out by optical method (Int. committee for standartization in haematology, 1988). The following parameters
were estimated: average size of aggregate (ASA), parameter of aggregation (PA) and percent of non aggregated erythrocytes
(PNE). For an estimation of structurally functional properties of membrane of erythrocytes were investigated
their cytoarchitecture. Patients data was processed statistically using SPSS version 14.
Results: Increase in ASA and PA (p > 0.05) is revealed in TS in comparison with the CG. In PD there was reliable
increase in (p < 0.05) contents of ASA and PA in I and II stages (6.2 ± 0.17; 6.1 ± 0.32), (1.7 ± 0.11; 1.6 ± 0.13)
accordingly and decrease in percent of PNE (58.6 ± 0.11) (p < 0.05) in stage I. Analysis of cytoarchitecture of erythrocytes
revealed that the main population of erythrocytes were cells of biconcave form (discocytes) and constituted 71.8
± 2.56 % in CG. Reversible forms of erythrocytes (RE) composed 18.2 ± 2.07 %, while IE: 9.69 ± 1.76 %. In children
with TS discocytes were 72.58 ± 1.66 %, RE – 13.84 ± 1.2 %, IE – 12.81 ± 1.28 % (p > 0.05). In patients with of PD,
contents of discocytes constituted 73.25 ± 3.01 % and 68.68 ± 4.14 % in stages I and II respectively, while IE were
15.5 ± 3.57 % in comparison with the CG (p > 0.05). Contents of RE constituted – 11.25 ± 1.6 %; and 11.37 ± 0.9 %
in I and II stages, p < 0.05 in comparison with the CG.
Conclusion: Rheological characteristics of erythrocytes in I - II stages of PD are indicated by authentic escalation in
aggregation of erythrocytes, reduction in reversible forms and increase in irreversible forms of erythrocytes at II stage
of disease. Changes in aggregation characteristics of erythrocytes in children with PD are reliably more expressive,
than in patients with TS. .
Keywords: Perthe’s` disease, rheological characteristics of erythrocytes, cytiarchitecture of erythrocytes, transient synovitis of the hip joint.
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