14. CHARACTERIZATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF UROPATHOGENS FROM KHYBER PAKHTUNKHWA, PAKISTAN

Fatima Gul1, Nafees Bacha1, Zahidullah Khan2, Sunia Arif Khan2, Awal Mir2, Ibne Amin3

1Department of Biotechnology & Microbiology, University of Peshawar - Pakistan
2Department of Pathology, Khyber Girls Medical College, Peshawar - Pakistan
3Department of Pathology, Kabir Medical College, Peshawar - Pakistan

Abstract

Objective: To characterize different uropathogens and determine their antibiotic susceptibility pattern.
Material and Methods: In the present study, 300 patients with clinical symptoms were investigated from tertiary care
hospitals of Khyber Pakhtunkhwa from January 2015 to December 2015. Clean catch midstream urine of patients were
collected, leukocyte count was determined and were cultured. The recovered bacteria were identified using different
biochemical tests. Susceptibility to antimicrobial agents was determined using disc diffusion method of Kirby-Bauer.
Results: From a total of 300 samples, 28.6% were positive for both bacterial culture and pus cells while 9.6% and
15.6% samples were having only pus cells and bacterial culture respectively. Microorganisms recovered and identified
were E. coli (73%) followed by Citrobacter freundii (9%) Staphylococcus aureus (4%), Pseudomonas aeruginosa (4%),
Morganella morganii (3%), Proteus, Streptococcus spp. and Candida albicans (1% each), while 4% were identified as
normal flora of the urinary tract. The recovered microorganisms were mostly sensitive to tazocin and amikacin while
they showed resistance against norfloxacin and tobramycin.
Conclusion: E. coli was the most common uropathogen. There is a strong association between pus cells and UTI. The
susceptibility pattern of different uropathogens show increasing resistance to commonly used antimicrobial agents.

Keywords: Urinary tract infection, pus cells, uropathogens.

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