BACTERIAL CULTURE ISOLATES FROM INFECTED DIABETIC FOOT TISSUE SPECIMENS AND THEIR SENSITIVITY TO ANTIMICROBIAL AGENTS

Romana Ayub1, Syed Shameer Raza1, Shafiullah2, Junaid Ahsan1, Amir Kamal Hussain1, Muhammad Daniyal Nadeem1

1Department of Community Medicine, Khyber Medical College, Peshawar - Pakistan
2Department of Community Medicine, Bacha Khan Medical College, Mardan - Pakistan

Abstract

Objective: To find out the most common organisms responsible for Diabetic Foot Infection (DFI) and their sensitivity to
antimicrobial agents for the prevention of sepsis/amputation by the administration of empirical treatment.
Material & Methods: Study was carried out to analyze the bacterial isolates of all patients admitted to the Surgical,
Medical and Orthopedic wards of KHYBER TEACHING HOSPITAL, Peshawar, Pakistan presented with diabetic foot
infection. The study period was from April 2016 to October 2016. We started by formulating a questionnaire that was
circulated among the designated groups of people, to check for organism responsible. Convenient sampling technique
is used. A 6 months long prospective study (taking the midyear population into account) was carried out. 100
patients having DFI (diabetic foot infection) were selected, their culture and sensitivity (C/S) reports were performed
and analyzed using SPSS 20.
Results: According to our findings a large number of people presenting with Diabetic Foot showed the following results:
A total of 62 (62%) aerobes and 38 (38%) fungal or anaerobes were isolated.
Conclusion: Staph aureus and E.coli are the most common Gram positive and Gram negative organisms, respectively,
in KPK. E.coli being the subset that mainly represents the bacterial population, isolated, upon culture, with high prevalence
of antimicrobial drug resistance particularly to Augmentin, cephradine, ciprofloxacin, cefutoxime and cefpodoxime
and sensitive to Cefoperazone/Sulbactam Vancomycin Imipenem and Piperacilline/Tazobectam.

Keywords: Culture, infection, diabetic foot, anti-microbial agents.

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